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10×Tris-glycine-SDS Running Buffer for SDS-PAGE

产品编号:T005

包装:100ml, 500ml, 5×500ml

价格:

T005

10×Tris-glycine-SDS running buffer


Properties

sterility   0.2 μm filtered
Featured Industry   Diagnostic Assay Manufacturing
storage temp.   2-8°C


Application

Tris-glycine-SDS (TGS) running buffer is the most commonly used buffer for sodium dodecyl sulfate – polyacrylamide gel electrophoresis (SDS-PAGE) of proteins. TGS is usually used for both the anode buffer and the cathode buffer. Recommended running conditions is 150 volts for mini vertical gel electrophoresis units.

Reconstitution

Dilution of the 10× TGS buffer produces a 1× running buffer containing 25 mM Tris, 192 mM glycine and 0.1% SDS, pH approx. 8.6.

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