产品编号:T005
包装:100ml, 500ml, 5×500ml
价格:
T005
10×Tris-glycine-SDS running buffer
Properties
sterility |
0.2 μm filtered |
Featured Industry |
Diagnostic Assay Manufacturing |
storage temp. |
2-8°C |
Application
Tris-glycine-SDS (TGS) running buffer is the most commonly used buffer for sodium dodecyl sulfate – polyacrylamide gel electrophoresis (SDS-PAGE) of proteins. TGS is usually used for both the anode buffer and the cathode buffer. Recommended running conditions is 150 volts for mini vertical gel electrophoresis units.
Reconstitution
Dilution of the 10× TGS buffer produces a 1× running buffer containing 25 mM Tris, 192 mM glycine and 0.1% SDS, pH approx. 8.6.
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