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XpresLytica Cell Lysis/Protein Extraction Reagent | 细胞裂解/蛋白抽提试剂,细胞裂解直通车 破菌试剂

产品编号:T102

包装:25ml,50, 100, 250ml

价格:199, 349, 648, 1299元

XpresLytica Cell Lysis /Protein Extract Reagent







细胞裂解直通车

10× Concentrated

适用于裂解细菌、培养的动物细胞、从组织或器官分离的细胞以释放出蛋白等生物大分子。本产品适用于少至几十微升细胞样品的裂解以满足快速少量制备蛋白样品的需要。特别是样品数量很少,无法用超声等方法破碎细胞时,本产品有突出优势。处理少量样品时,这个产品很好使。
强力促进蛋白,特别是疏水性蛋白的溶解。
与常用纯化方法兼容,不需要透析去除即可用原有条件进行层析
支持SDS-PAGE和2D电泳,样品不许去除破菌试剂即可直接电泳

请注意:室温低于20℃时,本产品有可能不能保持完全澄清体状态。届时,可在水浴锅或其他工具中适当加热使其为完全澄清状态并混匀后再使用。



产品编号

产品名称

规格

价格

规格

价格

规格

价格

规格

价格

T102

XpresLytica Cell Lysis Reagent

25ml

199

50ml

349

100ml

648

250ml

1299

Application

This product is suitable to extract proteins from bacteria and cultured animal cells, and compatible with any protein purification systems, including but not limited to affinity, ion exchange, hydrophobic chromatography. The detergent mix included in the XpresLytica Cell Lysis Reagent can be removed, if necessary, from the protein by washing during chromatography, by dialysis or ammonium sulfate precipitation. The final purity and yield of the recombinant product is usually higher than that obtained from classical traditional extraction methods. This is because the crude protein solution containing the non-ionic detergent eliminates much of the non-specific binding that occurs during chromatography. The mild detergent does not interfere with many enzyme assays or protein assays.

Features and Benefits

  • The 10×concentrated solution is compatible with all extraction buffers.
  • Suitable for both Gram positive and Gram negative bacteria, and also suitable for culture cells.
  • More efficient and quick than ultrasonication.
  • Fully compatible with affinity and other purification methods.
  • After soluble protein extraction, inclusion bodies can be isolated for subsequent solubilization.
  • Non-denaturing cell lysis preserves protein structure and function.

General description

This reagent is designed to efficiently lyses cells and extract proteins from both Gram negative and Gram positive bacteria that is difficult to lyses. This is accomplished using the standard ExpresLytica Cell Lysis Reagent , which is a proprietary blend of non-ionic detergents with several protease inhibitors and other components. Lysozyme (pae Cat No: C1359) and DigestAll (a Benzonase equivalent from pae, Cat No: C1280 ) can facilitate extraction efficiency and increase protein yield.
关键词:破菌试剂,破菌直通车,蛋白助溶剂,促溶,蛋白抽提,蛋白溶解,细胞裂解,蛋白2D-电泳,2D电泳,双向电泳。
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