首  页
核酸检测产品   首页 > 核酸检测产品
Agarose, for Electrophoresis, Proteomics Grade | 琼脂糖 电泳级

产品编号:C1732

包装:25G,50G,100G,250G;100G,250G,500G,

价格:660,1450,2200,5540;690,867,1989,3120





AGAROSE                           精品
琼脂糖
高品质 低价格 超高性价比
High strength and low EEO
for molecular biology, proteomics and cell culture

BioReagent, suitable for electrophoresis, southern bloting, northern bloting and western bloting, cell culture, etc.

CAT No Product Name Grade and Suitability Storage Package/Price
C1732A Agarose | 琼脂糖 BioReagent, Suitable for Proteomics, Cell Culture and Electrophoresis
EEO: 0.09~0.13; Gel Strength(1%) (g/cm2):≥1200; gel point 36.5 °C (1.5% gel, ± 1.5 °C)
RT 25G 660 50G 1450 100G 2200 250G 5540
C1732B Agarose | 琼脂糖 For Electrophoresis:
EEO:0.15; Gel Strength(1%) (g/cm2):≥950; Gelling Point (1%)(℃) 37±1.0
RT 100G 690 250G 1267 500G 1989 1KG 3120
 

Product Name: AGAROSE
CAS Number: 9012-36-6/ 39346-81-1
Melting point: 90–95 °C
Gelling point: 34–38 °C
EEO   0.09-0.13
Synonym: 3,6-Anhydro--L-galacto--D-galactan; FastLane agarose; Indubiose A4; NuSieve GTG; Odigose; Seakem; Sepharose

Suitability

Agarose is a preferred matrix for work with proteins and nucleic acids as it has a broad range of physical, chemical and thermal stability, and its lower degree of chemical complexity also makes it less likely to interact with biomolecules. Agarose is most commonly used as the medium for analytical scale electrophoretic separation in agarose gel electrophoresis. Gels made from purified agarose have a relatively large pore size, making them useful for separation of large molecules, such as proteins and protein complexes >200 kilodaltons, as well as DNA fragments >100 basepairs. Agarose is also used widely for a number of other applications, for example immunodiffusion andimmunoelectrophoresis, as the agarose fibers functions as an anchor for immunocomplexes.
As a gelling agent, agarose is used:
1. to separate nucleic acids electrophoretically because its gels have larger pore sizes than polyacrylamide gels at low concentrations. Unlike polyacrylamide, the consistency of the gels is more solid (but also less elastic);
2. To demonstrate cross reaction in IEP (Immuno electrophoresis) and Ouchterlony (double diffusion) plates in which antibody-antigen precipitin lines are studied;
3. to make gel plates or overlays for cells in tissue culture.
4. To form a gel matrix (either beaded and/or crosslinked) which can be used in chromatographic separations.

Properties

Agarose is commercially available as a white or yellowish powder or which dissolves in near-boiling water, and forms a gel when it cools. Agarose exhibits the phenomenon of thermal hysteresis in the liquid-to-gel transition, i.e. it gels and melts at different temperatures. The gelling and melting temperature varies depending on the type of agarose. Standard agaroses derived from Gelidium has a gelling temperature of 34–38 °C (93–100 °F) and a melting temperature of 90–95 °C (194–203 °F), while those derived from Gracilaria, due to its higher methoxy substituents, has a higher gelling temperature of 40–52 °C (104–126 °F) and melting temperature of 85–90 °C (185–194 °F). The melting and gelling temperature may be dependent on the concentration of the gel, particularly at low gel concentration of less than 1%. The gelling and melting temperature is therefore given at a specified concentration.
Natural agarose contains uncharged methyl groups and the extent of methylation is directly proportional to the gelling temperature. Synthetic methylation however have the reverse effect, whereby increased methylation lowers the gelling temperature. A variety of chemically modified agaroses with different melting and gelling temperatures are available; these are often made by hydroxyethylation of agarose.
On standing the agarose gels are prone to syneresis (extrusion of water through the gel surface), but the process is slow enough to not interfere with the use of the gel.
Agarose gel can have high gel strength at low concentration, making it suitable as an anticonvection medium for gel electrophoresis. Agarose gels as dilute as 0.15% can form slab for gel electrophoresis. The agarose polymer contains charged groups, in particular pyruvate and sulfate.[7] These negative charged groups can retard the movement of DNA in a process called electroendosmosis (EEO), and low EEO agarose is therefore generally preferred for use in agarose gel electrophoresis of nucleic acids.
The following is a list of properties associated with our agaroses :
Sulfate content :
used as an indicator of purity, since sulfate is the major ionic group present. Gel strength the force that must be applied to a gel to cause it to fracture.
Gel point
the temperature at which an aqueous agarose solution forms a gel as it cools. Agarose solutions exhibit hysteresis in the liquid-to-gel transition - that is, their gel point is not the same as their melting temperature. 
Electroendosmosis (EEO) 
a movement of liquid through the gel. Anionic groups in an agarose gel are affixed to the matrix and cannot move, but dissociable counter cations can migrate toward the cathode in the matrix, giving rise to EEO. Since electrophoretic movement of biopolymers is usually toward the anode, EEO can disrupt separations because of internal convection.

Specification

Grade BioReagent
impurities   ≤10% moisture content
EEO   0.09-0.13
transition temp gel point 36 °C (1.5% gel, ± 1.5 °C)
gel strength   ≥1200 g/cm2 (1% gel)
foreign activity DNase, RNase, none detected
 

版权所有 无锡拼搏官网app有限公司 苏ICP备12045354 
地址:无锡 • 惠山 •  智慧路33号华清创意园7栋601室   电话:0510-81819585 手机:189 5157 6701 QQ:2642 166682